Development of biopolymer nanocomposite for silver nanoparticlesand Ciprofloxacin controlled release

ISLAN GERMAN A; MUKHERJEE ARUP; CASTRO GUILLERMO R

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 72 p. 740 - 750

0141-8130

tScreening of biopolymeric gel beads containing Silver NanoParticles (Ag-NPs) stabilized in Guar GumAlkyl Amine (GGAA) and Ciprofloxacin (Cip) was carried out in order to obtain a novel nanocompositewith controlled release profile of both antimicrobians. The selected matrix composed of Alginate/HighMethoxyl Pectin (HMP)/GGAA (4:4:1) was able to co-incorporate Ag-NPs and Cip with encapsulationefficiency higher than 70%. SEM images revealed good cohesivity and compatibility between the biopoly-mers and the cargos. Beads provided protection against Ag-NPs degradation at acidic pHs and HMP wouldplayed a key role providing hydrophobic regions. While Cip release profile showed a pH independent dif-fusional process, Ag-NPs release was restricted to matrix erodability. Calcium quelating agents and/oralginate degrading enzymes could modulate the release profile. The bactericidal activity of beads wastested in liquid medium, showing cooperative effects between the antimicrobials against Pseudomonasaeruginosa, Escherichia coli, Bacillus cereus and Staphylococcus aureus. TEM images confirmed interactionof Ag-NPs with bacterial surfaces followed by membrane damage. Results suggested the nanocompositematrix as a promising system for oral treatment of intestinal infectious diseases caused by multidrugresistant and unknown microorganisms, since both Cip and Ag-NPs would be able to reach intestine inthe active form.