CINDEFI   05381
CENTRO DE INVESTIGACION Y DESARROLLO EN FERMENTACIONES INDUSTRIALES
Unidad Ejecutora - UE
artículos
Título:
Profiling the Bordetella pertussis proteome during iron starvation.
Autor/es:
PEREZ VIDAKOVICS M L; PABA J; LAMBERTI Y; RICART C A; VALLE DE SOUSA M; RODRIGUEZ M E
Revista:
Journal of Proteome Research
Referencias:
Año: 2007 vol. 6 p. 2518 - 2528
ISSN:
1535-3893
Resumen:
Regulation of gene expression in response to local iron concentration
is commonly observed in bacterial pathogens that face this nutrient
limitation during host infection. In this study, a proteomic approach
was used to analyze the differential protein expression of Bordetella
pertussis under iron limitation. Whole cell lysates (WCL) and outer
membrane fractions of bacteria grown either under iron-starvation or
iron-excess conditions were analyzed by two-dimensional (2-D) gel
electrophoresis. Statistical analysis revealed 36 proteins displaying
differential expression, 9 with higher expression under iron-excess and
27 with increased expression under iron-starvation. These proteins were
subjected to tryptic digestion and MALDI-TOF MS. Apart from those
previously reported, we identified new low-iron-induced proteins that
might help to explain the increased virulence of this phenotype.
Additionally, we found evidence that at least one of the identified
proteins, solely expressed under iron starvation, is highly immunogenic
in infected individuals.