CINDEFI   05381
CENTRO DE INVESTIGACION Y DESARROLLO EN FERMENTACIONES INDUSTRIALES
Unidad Ejecutora - UE
artículos
Título:
Evaluation of biofilm-forming capacity of Moraxella bovis, the primary causative agent of infectious bovine keratoconjunctivitis
Autor/es:
PRIETO C; SERRA DO; MARTINA P; JACOBS M; BOSCH A; YANTORNO OM
Revista:
VETERINARY MICROBIOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2013 vol. 166 p. 504 - 515
ISSN:
0378-1135
Resumen:
The difficulties in preventing and treating infectious bovine keratoconjunctivitis (IBK) and
the consequent impact on the cattle industry worldwide emphasize the need to better
understand this infectious process along with the biology of Moraxella bovis, its primary
causative agent. Although there is increasing evidence that bacterial biofilms participate in
a variety of ocular infections by direct biofilm formation on the surfaces of the eye, IBK has
not been considered as a biofilm-based disease so far, and even more, no information is
currently available regarding the ability of M. bovis to adopt a biofilm lifestyle. In the
present research, we demonstrated the capacity of M. bovis clinical isolates and reference
strains to form biofilms on different abiotic surfaces and culture conditions, and provided
qualitative and quantitative information on the biofilm growth and architecture of mature
biofilms. In addition, our data indicated that the type IV pili play a critical role in the
biofilm formation in vitro. Most significantly, we proved that through exposure to MgCl2
type IV pili are removed from the cell surface, not only preventing M. bovis biofilm
formation but also disassembling preformed biofilms. These results could constitute a new
approach in the understanding of M. bovis colonization process in cattle eye and/or nasal
cavity, and may aid in the development of future antimicrobial strategies for the control of
IBK.