Alginate lyase and ciprofloxacin co-immobilization on biopolymeric microspheres for cystic fibrosis treatment.

ISLAN GERMAN A; BOSIO VALERIA E.; CASTRO GUILLERMO R

MACROMOLECULAR BIOSCIENCE

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2013 vol. 13 p. 1238 - 1248

1616-5187

Opportunistic pathogens such as Pseudomonas aeruginosa have been found in the respiratory and intestinal tracts of cystic fibrosis patients and can be hardly eliminated by using only antibiotics, due to the presence of dense mucus covering organs surface mainly composed of alginate. The aim of the present work is to develop a new formulation based on biopolymeric microspheres containing Alginate Lyase (AL, enzyme able to hydrolyze alginate) and Ciprofloxacin (Cip, a fluoroquinolone antibiotic) for sustainable oral delivery in cystic fibrosis patients. Alginate (ALG) and High Methoxyl Pectin (HMP) were selected as the biopolymers to develop a composite matrix, due to its desirable properties on controlled release of fluoroquinolones. ALG microspheres coated with HMP and ALG-HMP blend were gelled in water-organic solvents mixtures, obtaining Cip encapsulation percentages ranging from 46.0 to 100.0 %. The ALG-HMP showed a Cip sustainable release profile and was able to encapsulate 90.0 % of AL, showing 76.0 % enzyme activity after release under simulated intestinal conditions. The developed system is a promising delivery carrier to treat chronic infection of Pseudomonas aeruginosa and to reduce the viscoelasticity of the mucus accumulated into intestine of cystic fibrosis patients, improving their life quality.