One-step concentration and partial purification of Aspergillus kawachii non-acidic polygalacturonases by adsorption to glass fiber microfilters

CLAUDIO E. VOGET, CAROLINA E. VITA, JUAN C. CONTRERAS ESQUIVEL

BIOTECHNOLOGY LETTERS

SPRINGER

Año: 2006 vol. 28 p. 233 - 239

0141-5492

The non acidic polygalacturonases produced by Aspergillus kawachii in a glucose-tryptone medium were adsorbed to a glass fiber microfilter that was used to clarify the fermentation broth. Maximum adsorption occurred at pH 3.0 under low ionic strength conditions. The adsorbed activity could be readily released with a buffer solution at pH 5.0. Based upon these observations it was developed a separation process which enabled  the broth to be clarified and, at clarification and at the same time the non acidic polygalacturonases to be concentrated 20-fold at the same time, the non-acidic polygalacturonases to be concentrated 20-fold and purified 100-fold in a unique filtration step. The practical advantage of recovering  polygalacturonases by a filtration process lies in the simplicity and efficiency of the operation involved.