EFFECTS OF THE INOCULANT STRAIN SPHINGOMONAS PAUCIMOBILIS 20006FA ON SOIL BACTERIAL COMMUNITY AND BIODEGRADATION IN PHENANTHRENE CONTAMINATED SOIL.

COPPOTELLI BIBIANA M.; IBARROLAZA AGUSTÍN; DEL PANNO MARÍA T.; MORELLI IRMA S.

MICROBIAL ECOLOGY

Springer Science

Lugar: New York; Año: 2007

0095-3628

 The effects of the inoculant strain Sphingomonas paucimobilis 20006FA (isolated from a phenanthrene contaminated soil) on the dynamics and structure of microbial communities and phenanthrene elimination rate were studied in soil microcosms artificially contaminated with phenanthrene. The inoculant managed to be established from the first inoculation as it was evidenced by denaturing gradient gel electrophoresis (DGGE) analysis, increasing the number of cultivable heterotrophic and PAH-degrading cells and enhancing phenanthrene degradation. These effects were observed only during the inoculation period. Nevertheless, the soil biological activity (dehydrogenase activity and CO2 production) showed a late increase.  While gradual and successive changes in bacterial community structures were caused by phenanthrene contamination, the inoculation provoked immediate, significant and stable changes on soil bacterial community. In spite of the long-term establishment of the inoculated strain, at the end of the experiment the bioaugmentation did not produce significant changes in the residual soil phenanthrene concentration and did not improve the residual effects on the microbial soil community.