CINDEFI   05381
CENTRO DE INVESTIGACION Y DESARROLLO EN FERMENTACIONES INDUSTRIALES
Unidad Ejecutora - UE
artículos
Título:
Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
Autor/es:
CHESINI, M.; NEILA, L.P.; FRATEBIANCHI DE LA PARRA, D.; ROJAS, N.L.; CONTRERAS ESQUIVEL, J.C.; CAVALITTO, S.F.; GHIRINGHELLI, P.D.; HOURS, R.A.
Revista:
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
Editorial:
UNIV CATOLICA DE VALPARAISO
Referencias:
Lugar: Valparaíso; Año: 2013 vol. 16 p. 1 - 13
ISSN:
0717-3458
Resumen:
Inulinases have been extracted and characterized from inulin-storing
tissues; however, production of microbial inulinases has recently drawn much
attention as they offer several industrial advantages. Many microorganisms,
including filamentous fungi, yeast and bacteria have been claimed as inulinase
producers. These hydrolases are usually inducible and their exo-acting forms
may hydrolyze fructose polymers (inulin) and oligosaccharides such as sucrose
and raffinose. Fungal inulinase extracts are often produced as stable mixture
of highly active fructanhydrolases. From a practical prospective, the best
known inulinases to date are those produced by species of Penicillium,
Aspergillus and Kluyveromyces.
The production of extracellular inulinase by A. kawachii in
liquid cultures, using either inulin or yacon derived materials as CES as well
as inulinase inducers, is reported. In addition, a partial characterization of
the enzyme activity is included.
Yacon derived products, particularly yacon juice,
added to the culture medium proved to be a good CES for fungal growth as well
as an inducer of enzyme synthesis. Partial characterization of the enzyme
revealed that it is quite stable in a wide range of pH and temperature. In
addition, characterization of the reaction products revealed that this enzyme
corresponds to an exo-type. These facts are promising considering its potential
application in inulin hydrolysis for the production of high fructose syrups.