Enzymatic synthesis of 4-pentulosonates (4-keto-D-pentonates) from D-aldopentose and D-pentonate by two different pathways using membrane enzymes of acetic acid bacteria

ADACHI, O.; HOURS, R.A.; SHINAGAWA, E.; AKAKABE, Y.; YAKUSHI, T.; MATSUSHITA, K.

BIOSCIENCE, BIOTECHNOLOGY AND BIOCHEMISTRY

JAPAN SOC BIOSCI BIOTECHN AGROCHEM

Lugar: Tokyo; Año: 2011 vol. 75 p. 2418 - 2420

0916-8451

4-Keto-D-arabonate (D-threo-pent-4-ulosonate) and 4-keto-D-ribonate (D-erythro-pent-4-ulosonate) were prepared from D-arabinose and D-ribose by two successive reactions of membrane-bound enzymes, D-aldopentose 4-dehydrogenase and 4-keto-D-aldopentose 1-dehydrogenase of Gluconobacter suboxydans IFO 12528. Alternatively, they were prepared from D-arabonate and D-ribonate with another membrane-bound enzyme, D-pentonate 4-dehydrogenase. Analytical data confirmed the chemical structures of the 4-pentulosonates prepared. This is the first report of successful enzymatic synthesis of 4-pentulosonates.