IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Nanoberries: further characterization of liposomal polyphenols and in vitro photoprotection against UV attack
Autor/es:
BUCCI, PAULA L; MONTANARI, JORGE A.M.; SILVIA DEL VALLE ALONSO
Lugar:
Sierra de la Ventana
Reunión:
Congreso; SAB 2014 - XLIIi Reunión anual de la Sociedad Argentina de Biofísica; 2014
Institución organizadora:
Sociedad Argentina de Biofísica
Resumen:
Nanoberries1 are ultradeformable liposomes
loaded with an ethanolic extract rich in polyphenols from blueberry (Vaccinium myrtillus, variety Millenia).
They are potentially useful as delivery systems for photoprotection against
UV-mediated skin damage. We prepared the liposomes with soy phosphatidylcholine
and sodium cholate as a border activator, at 0.223 extract/lipid w/w. Their phenolic
and anthocyanine content, drug to lipid ratio, size and Zeta potential after
manual extrusion were determined, and we obtained images by Scanning Electron
Microscopy after drying the Nanoberries under different conditions:
lyophilization and vacuum dry2. With the aim of assessing their
photoprotection capacity against UV attack, we performed experiments on HaCaT
cells. A calibration curve of phototoxicity was obtained by viability
determination after exposure to different amounts of energy from an UV source. Then,
the protection against phototoxicity by Nanoberries and free ethanolic extracts
of blueberry were assessed.
Acknowledgements:
We thank ?The Berry Store? for kindly providing blueberries for this research
work, and Dr. Rebeca Oliveira de Souza (Fcfrp, USP, Brazil) for her help with
phototoxicity assays.
References
1 Montanari et al.
Journal of Cosmetic Sciences. 2013. 469.
2 Montanari et al.
International Journal of Pharmaceutics. 2009. 184.