Distribution and localization of neuronal sets that co express growth hormone secretagogue receptor and Glucagon like Peptide 1 Receptor in the Mouse Brain

FERNANDEZ GIMENA; CORNEJO PAULA; AGUGGIA JULIETA P.; BARRILE FRANCO; ,ABDELLA M. HABIB; CASSANO, DANIELA; SAEZ CAMILA

Buenos Aires

Congreso; Sociedad Argentina de Investigación en Neurociencias; 2022

Ghrelin is a stomach derived orexigenic hormone that acts via the growth hormone secretagogue receptor ( highly expressed in the brain that increases food intake and glycemia The Glucagon like peptide 1 (GLP 1 is a hormone released by the gastrointestinal tract in response to meal intake that acts via the GLP 1 receptor (GLP 1 R) and reduces food intake and glycemia Interestingly GHSR and GLP 1 R expression have been observed within many of the same nuclei of the brain, suggesting may act on common neuronal sets to mediate its neurobiological effects Here, we explored the extent of this putative direct. GHSR and GLP 1 R interaction in the brain. We mapped the distribution of the GHSR in the mouse brain and examined the localization of this receptor using two complementary approaches binding with fluorescent labeled ghrelin (Fr ghrelin) in wild type mice or visualizing the endogenous fluorescence of GHSR eGFP mice in which eGFP is under the control of the GHSR promoter In both cases, the presence of GLP 1 R was visualized by immunohistochemistry using a validated anti GLP 1 R antibody