MRP4 is a metastatic enhancer in pancreatic cancer

MAXIMILIANO DE SOUSA SERRO; CARINA SHAYO; MARÍA MAY; NICOLAS DI SIERVI; ANA SAHORES; NATALIA GOMEZ; CARLOS DAVIO

San Carlos de Bariloche

Congreso; SISTAM; 2018

SISTAM

Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal human malignancies, in part due to its propensity for metastasis. There is an urgent need of novel strategies to target PDAC and improve its prognosis. Recent evidence from our lab shows that pancreatic cancer cell lines and tumors express higher levels of MRP4 compared to adjacent tissue. This transporter proved to be critical for PDAC cell proliferation and tumorigenicity. The aim of this study was to evaluate MRP4 role in pancreatic cancer metastasis. Panc-1 PDAC cells were stably transfected with scramble or shMRP4 vectors and the scratch-wound assay was used to assess migration. Wound closure was significantly slower in the silenced clones compared to the scramble cells.MRP4 expression level of PDAC primary tumors, adjacent tissue, hematological and circulatory tumor cells (CTC) was obtained from GEO:GSE18670 dataset. Whole genome microarray analysis showed that CTC cells depict higher MRP4 levels compared to normal (p