Differential β2-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines

GARGIULO LUCÍA; COPSEL SABRINA; RIVERO EZEQUIEL; GALÉS CÉLINE ; SÉNARD JEAN MICHEL; LUTHY ISABEL; DAVIO CARLOS; BRUZZONE ARIANA

Oncotarget

Impact Journals

Lugar: Albany, N.Y.; Año: 2014 vol. 5 p. 10058 - 10069

1949-2553

Breast cancer is the most frequent malignancy in women. Several reportsdemonstrated that adrenergic receptors (ARs) are involved in breast cancer. Herewe observed that epinephrine (Epi), an endogenous AR agonist, caused oppositeeffects in non-tumorigenic (MCF-10A and HBL-100) and tumor cells (MCF-7 and MDAMB-231). Thus, Epi, in non-tumor breast cells, as well as isoproterenol (β-agonist),in all cell lines, maintained a benign phenotype, decreasing cell proliferation andmigration, and stimulating cell adhesion. β-AR expression and cAMP levels werehigher in MCF-10A than in MCF-7 cells. β2-AR knock-down caused a significantincrease of cell proliferation and migration, and a decrease of cell adhesion both inbasal and in Iso-stimulated conditions. Coincidently, β2-AR over-expression induceda significant decrease of cell proliferation and migration, and an increase of celladhesion. Therefore, β2-AR is implied in cell phenotype and its agonists or antagonistscould eventually complement cancer therapy.