Evaluation of muscarinic receptors silencing by specific RNA interference on migration and angiogenesis in human breast cancer cells

GIAMBALVO GÓMEZ D; MARTÍNEZ PULIDO P; MARTÍNEZ PÉREZ A.M; LOMBARDI MG.; OROÑO M

Mar del Plata

Congreso; Reunión anual de la Sociedad Argentina de Investigación Clínica; 2018

Sociedad Argentina de Investigación Clínica

It has been reported that muscarinic receptors (M) are absent in normal breast cells and are up-regulated in tumor cells. Particularly, MCF-7 cells express M3 and M4 subtypes and its activation promotes tumoral progression. We demonstrated that M expression in non-tumorigenic human mammary cell lines triggers malignant transformation. RNA interference (RNAi) is a non-invasive specific technology and in the last years different groups are working in RNAi based therapeutics. Here, we analyzed the effect of RNAi gene silencing of M3 and/or M4 receptors in human breast cancer MCF-7 cells (siM3M4) on migration activity in vitro by wound healing assay and on induced angiogenesis in vivo. We previously reported that the treatment with the cholinergic agonist carbachol(10-8M) significantly increased the migration capacity of MCF-7 cells (195±4.8% vs control (cell without treatment considered as 100%) p