A NEW ROLE FOR LYSOPHOSPHATIDIC ACID IN VASCULAR PROCESSES AT THE MATERNAL-FETAL INTERFACE DURING EARLY GESTATION.

SORDELLI MS; FRANCHI AM; BELTRAME JS; SCOTTI L; RIBEIRO ML; CAÑUMIL VA; PARBORELL F

Buenos Aires

Congreso; Reunión Conjunta de las Sociedades de Biociencias; 2017

SAFIS

Successful implantation requires that the invading trophoblast acquires an endovascular phenotype and remodels uterine spiral arteries. Defects in this mechanism correlate with obstetric complications as implantation failure and preeclampsia. Lysophosphatidic acid (LPA) participates in embryo implantation and contributes to vascular physiology in different biological systems. However, the role of LPA on vascular processes at the implantation site has not been investigated. We adopted an in vitro and an in vivo pharmacological approach to study LPA action on trophoblast endovascular response and uterine transformation. The HTR-8/SVneo cell line (H8) was used to model the acquisition of the trophoblast endovascular phenotype. LPA (10 µM) increased H8 tube formation (6h), migration (wound healing, 18h) and proliferation (MTT assay, 48h) (p