Effect of dehydroepiandrosterone and metformin in the mouse corpus luteum regression

SANDER VA; FACORRO G; PIEHL L; RUBIN DE CELLIS E; MOTTA AB

REPRODUCTION

Bioscientifica

Año: 2008

1470-1626

ESTE TRABAJO ESTA ENVIADO, AUN NO ACEPTADO PARA SU PUBLICACION.  -Polycystic ovary syndrome (PCOS) is characterized by hyperandrogenemia, hirsutism, oligo- or amenorrhea and anovulation. Hyperandrogenism alters ovarian function leading to abnormal length of estrus cycles, anovulation and high rates of recurrent miscarriages. The aim of the present study was to investigate the action of androgens on corpus luteum (CL) regression and the mechanism by which N, N- dimethylbiguanide metformin is used to restore cyclicity. The injection of dehydroepiandrosterone (DHEA; 60 mg/Kg body weight) 24 and 28 before luteolysis to BALB/c mice increased serum progesterone and estradiol levels and decreased ovarian oxidative stress since it decreased lipid peroxidation, increased both superoxide dismutase and catalase activities and the total capacity to scavenge ·OH. Therefore, the treatment with DHEA diminished the ovarian production of the luteolytic prostaglandin (PG) F2 alpha and increased the ovarian levels of the luteotrophic PGE. Therefore, DHEA increased ovarian nitrite concentration. The effects of DHEA on both PGs and NO production involved the regulation of the corresponding main enzymes of each pathway. We conclude that hyperandrogenism delays the regression of CL in mice.