Molecular basis of hyposensitivity ligth responses in Arabidopsis plant from Patsgonie, Argentina.

RUIZ DIAZ MARIA JIMENA; BETH ROWAN; DANIELE FILIAULT; JAVIER FRANCISCO BOTTO

Foz do Iguazu

Congreso; 11 Congress of International Plant Molecular Biology.; 2015

IPMB

Molecular basis of hyposensitivity light responses in Arabidopsis plants from Patagonia, ArgentinaMaría Jimena Ruiz Diaz1, Beth Rowan2, Daniele Filiault3, Javier F. Botto41IFEVA, Facultad de Agronomía-UBA-CONICET, Buenos Aires, Argentina. 2Max Planck Institute for Developmental Biology, Tübingen, Germany. 3Gregor Mendel Institute, Vienna, Austria.The model plant, Arabidopsis thaliana, has a native range throughout Eurasia and North Africa. Recently, introduced populations have been collected in South America within a ~3500-km2 area in Patagonia, Argentina, and whole-genome sequencing revealed that Patagonia (Pat) plants formed a single haplogroup. The Pat haplogroup is hyposensitive to red light during de-etiolation, and displays reduced shade avoidance responses. We previously identified that polymorphisms in the PHYB and BBX4 genes were associated with light hyposensitivity. The aim of this work was to gain insights into PHYB signaling by studying the polymorphic basis of light responses in Pat and related genotypes. Using physiological and genetic approaches, we found that a) haplotype analysis of PHYB suggested that Pat is part of a rather large and widely distributed ?old? haplogroup consisting of 186 accessions that are well represented in Iberia; and b) accessions sharing 100% of similarity with Pat in the PHYB gene show a good correlation with the hyposensitivity of light responses. We will examine the relative contribution of PHYB polymorphisms to different light responses by generating whole PHYB gene sequences, constructing transgenic plants with different PHYB polymporphisms and by cosegregation analysis.