Reconstruction of Anaerolineae genomes (Phylum Chloroflexi) from metagenome datasets in a full scale UASB methanogenic reactor

ERIJMAN, LEONARDO; GUERRERO, LEANDRO D.; ETCHEBEHERE, CLAUDIA; BOVIO-WINKLER, PATRICIA; CABEZAS, ANGELA

Congreso; The Latin American Meetings on Anaerobic Digestion; 2020

The Anaerolineae class within phylum Chloroflexi is reported as one of the predominant groups in anaerobicreactors. As they are difficult to obtain in pure culture, very little is known about their role in these systems.From the information retrieved from 7 isolated members and 3 assembled genomes recovered from differentwastewater treatment systems, it was reported that they ferment carbohydrates and have a slow growth rate. Dueto their filamentous morphology it has been suggested that they play an important role in the formation ofgranules in UASB reactors, a fundamental process for biomass retention. However, the overgrowth ofAnaerolineae members has been related to bulking processes. The objective of the present work was to assemblenew genomes of members of Anaerolineae from metagenomes obtained from samples of an UASBmethanogenic reactor from a UASB reactor treating malt processing wastewater. Four genomes belonging to thisclass were assembled with a completeness between 86.36% and 92.73%. The average nucleotide identity (ANI)percentages were between 70 and 84%, therefore the assembled genomes represent new species. All genomespresented the potential of anaerobic respiration and fermentative metabolism.