High quality draft genomes of members of acidobacteria subdivsion 4resolved from two industrial wastewater treatment plant metagenome

CAROL DAVIES SALA; FEDERICO MATIAS IBARBALZ; LEONARDO ERIJMAN; LEANDRO GUERRERO; EVA FIGUEROLA; ESTEBAN ORELLANA

Buenos Aires

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Sociedad Argentinas de Biociencias (SAIB, SAIC, SAI, SAA, SAB, SAB, SAFE, SAFIS, SAH, SAP)

The activated sludge (AS) technology, used worldwide for thetreatment of municipal and industrial wastewater, relies on theself-assembly of a highly diverse and dynamic microbial community,where bacteria are responsible for the removal of most of the oxygen-demanding pollutants and nutrients. Our general aim is to gainunderstanding of AS functioning through the ecophysiology of keymicrobial players.Shotgun sequencing of samples taken at different time from theaeration basins of two industrial full-scale wastewater treatmentplant (WWTP), textile-dyeing (TD) and polymer synthesis (PS),was performed using Illumina HiSeq 1500. A total of 4.38 x 107 highquality paired end reads of 150bp (6.57 x 109 bp) were assembled.Differential coverage of scaffolds was used as a binning strategy,followed by %GC and tetranucleotide frequency. Paired end readswere tracked and reassembled, and contamination with foreign genomeswas checked.Out of forty-five genomes assembled into near-complete chromosomes,we focused primarily on two genomes of a stable andrelatively abundant (ca. 9.8 % and 6.8%) member of this community,according to PCR amplification based on a specific region ofthe 16S rRNA gene and in silico abundance estimation. The genomefrom TD had 3.6 Mbp and 92.3% of completeness with 0.8%of contamination, while the genome from the PS had 2.7 Mbp with77.2% of completeness and 0.05% of contamination. Phylogeneticreconstruction using 16S rRNA and 26 conserved genes sequencesindicated both genomes belonged to Acidobacteria Subdivision 4,Blastocatellaceae family.The resolved genomes are distinguished by the relatively high proportionof genes coding for enzymes related to membrane transport(such as type VI secretion machine) and osmotic stress response(EnvZ-OmpR). These features are consistent with the ability ofthese bacteria to thrive in a highly competitive, nutrient poor environmentof high salt concentration.