Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae

WEHRENDT DIANA PATRICIA; TELLEZ-IÑÓN MARIA TERESA; FERRER MARÍA JOSÉ; COMINI MARCELO; BONILLA MARIANA; POTENZA MARIANA

T. cruzi Infection - Methods and Protocols

Humana Press

Año: 2019; p. 105 - 118

Trypanosomatids are unicellular organisms that colonize a wide diversity of environments and hosts. For instance, Trypanosoma cruzi is a human pathogen responsible for Chagas diseases, while Leishmania tarentolae infects amphibians and became a biotechnological tool suitable for recombinant protein expression.T. cruzi antigens are needed for the development of improved epitope-based methods for diagnosis and treatment of Chagas disease. Molecular cloning for the production of recombinant proteins offers the possibility to obtain T. cruzi antigens at high yield and purity. L. tarentolae appears as the ideal expressionhost to obtain recombinant T. cruzi antigens with a structure and posttranslational modifications typical of trypanosomatids. In this chapter, we present a protocol for the analytical to mid-scale production of recombinant T. cruzi antigens, using L. tarentolae as expression host (LEXSY® inducible system).