EFFECT OF THE PRESENCE OF A MAMMARY TUMOR IN ANIMALS SUBMITTED TO A HIGH-FAT DIET

CHIARALUCE, F; BARRANCO, MM; ZECCHINATI, F; CILENZO, A; ROZADOS, VIVIANA; RICO, MARIA JOSE; VILLANUEVA, SSM; SCHAROVSKY O, GRACIELA; MAINETTI LEANDRO; GARCÍA, FABIANA

Rosario

Congreso; XIX CONGRESS ? XXXVII ANNUAL MEETING SOCIEDAD DE BIOLOGIA DE ROSARIO; 2017

SOCIEDAD DE BIOLOGIA DE ROSARIO

Epidemiologic studies indicate that consumption of a high-fat diet (HFD) may increase the risk of invasive breast cancer in posmenopausal women. P-glycoprotein (P-gp) is an efflux pump of the intestinal epithelium. We aimed to evaluate in mice submitted to a HFD the effect of the presence of a mammary tumor. Five weeks old BALB/c mice were fed with a 40% bovine fat diet (Group HFD; n=10) and with standard diet (Control Group C; n=10). Body weight (BW) was weekly assessed. A glucose tolerance curve was developed at week 12 and half of the mice in each group were challenged s.c. with triple negative M-234p mammary adenocarcinoma (HFD+T, n=5; C+T, n=5). Tumor size was evaluated 3 times/week and, at week 16, an insulin tolerance test (ITT) was done and samples of serum obtained; mice were euthanized and epididymal fat and jejunal mucosa samples were taken. Body weight gain, with higher values in HFD groups, showed a diet (P