SEXUAL DIMORPHISM OF CELLULAR AND HUMORAL IMMUNITY IN EXPERIMENTAL VACCINES AGAINST TRYPANOSOMA CRUZI

BULFONI BALBI CAMILA; DINATALE BRENDA; PACINI MARÍA FLORENCIA; GONZÁLEZ FLORENCIA BELÉN; VILLAR SILVINA; PROCHETTO ESTEFANÍA; FARRÉ CECILIA; CHAPO GUSTAVO; MARCIPAR IVÁN; CABRERA, GABRIEL; PÉREZ ANA ROSA

Congreso; Reunión Conjunta SAIC. SAI. AAFE. NANOMED-AR; 2021

There is evidence that vaccine efficacy is affected by diverse parameters like sex between other. Thus, the inclusion of both sexes in studies of immune function becomes increasingly imperative. Usually, vaccine research against T. cruzi (Tc) has been focused on antibody response evaluation. Thus, we aim to evaluate possible variations in humoral and cellular response induced after experimental vaccine administration against Tc between BALB/c males (M) and females (F). Thus, mice (n=5/group) were immunized intranasally [3 doses, one every 2 weeks with 10µg of TS emulsified in ISPA (I) or c-di-AMP (A) adjuvants]. Controls were treated with saline (S) or TS alone. Fifteen days after the last immunization, in vivo cell-mediated (delayed hypersensitivity test -DHT-) and specific humoral (ELISA) responses were assayed. Later, mice were orally challenged with 3000 Tc (sub-lethal challenge). Parasitemia and clinical score were evaluated until day 100 post-infection (pi). In both M and F, the DHT was higher in TS+I and TS+A groups compared to their respective controls (p