Evolution of LysM-RLK family in wild and cultivated peanut species

JOHAN STIBEN RODRIGUEZ MELO; MARÍA CAROLINA BARBOSA; FEDERICO ARIEL; JIANPING WANG; ADRIANA FABRA; M.L. TONELLI; FERNANDO IBAÑEZ

Congreso; XXXIII Argentinian Meeting of Plant Physiology; 2021

SAFV

LysM-RLKs are transmembrane proteins containing three extracellular LysM domains, a transmembrane region and an intracellular kinase domain. These proteins are exclusively found in plants and are crucial for differentiation among structurally similar signaling microbial molecules or patterns. In legumes, LysM-RLK perception of rhizobial Nod factors triggers a signaling pathway related to the onset of symbiosis development. On the other hand, activation of LysM-RLKs upon recognition of chitin-derived short-chitooligosaccharides initiates defense responses. In this work, we identified the members of LysM-RLK gene family in cultivated (A. hypogaea L.) and wild (A. duranensis and A. ipaensis) peanut genomes and reconstructed their evolutionary history. Within this phylogenetic framework, we reinterpreted the functional data reported for peanut LysM-RLKs. In addition, potential roles of two identified proteins during Nod factors and chitosan inoculation were assessed by gene expression analyses and protein modelling. Results indicated that peanut LysM-RLK is a highly diverse family. Digital expression analyses indicated that specific A. hypogaea LysM-RLK receptors were upregulated during early and late stages of symbiosis. Also, expression profiles and 3D protein folding analysis predictions of selected receptors revealed particularities possibly related to their capacity to recognize Nod factors and/or chitosan.Analyses of LysM-RLK in the non-model legume peanut can contribute to gain insight into the molecular basis determining legume-microbe interactions and to the understanding of the evolutionary history of this gene family within the Fabaceae.