BECAS
PALMA NicolÁs Francisco
congresos y reuniones científicas
Título:
STUDY OF CONDUCTUAL PARAMETERS IN SPONTANEOUS DIABETIC RATS (eSS) AT DIFFERENT AGES AND THEIR POSSIBLE RELATIONSHIP WITH MORPHOLOGICAL ALTERATIONS OF THE HIPPOCAMPUS
Autor/es:
PALMA NICOLÁS; CHOLICH VALERIA; GVOZDENOVICH MARTÍN; FUNES ALEJANDRINA; ALLASIA BELÉN; CHAPO GUSTAVO; PACCHIONI ALEJANDRA; QUINTANA ALEJANDRA
Lugar:
ROSARIO
Reunión:
Congreso; XXI Congreso y XXXIX Reunión Anual de la SOCIEDAD DE BIOLOGÍA DE ROSARIO; 2019
Institución organizadora:
SOCIEDAD DE BIOLOGÍA DE ROSARIO
Resumen:
Type 2 Diabetes Mellitus (DM2) produces metabolic disorders that can induce cognitive and motor disturbances. DM has beenrecognized in many mammals, which provide useful experimental models. A line of spontaneous diabetic rats called eSS is producedat CIPReB. In previous work, we observed disorders in the explicit memory of eSS rats. We studied the progressive effect of DM2 onbehavior, as well as to evaluate the presence of possible morphological alterations in the hippocampus of eSS rats. 12eSS and12Wistar rats (W, non-diabetic controls) were studied, between postnatal day 80 (DPN80) and 270. Once a month, we evaluated:spontaneous motor activity (open field), anxiety levels (open field and light-dark test), and motor coordination (rotating roller). Tomeasure the total motor activity and the time spent in the central area of the open field (measure of anxiety) square acrylic boxes wereused. In the light-dark test, acrylic boxes had one-half black and the other half white, divided by a wall with an opening in the base thatallowed the animal to pass from one side to the other. To assess motor coordination, the animals were placed on a rotating roller andthe time they could stay on it was measured. After the tests, the rats were anesthetized and subjected to intracardiac perfusion withparaformaldehyde. The brains were extracted and coronal cuts (40 µm) were obtained, which were stained with Congo Red to identifythe presence of amyloid substance (SA) in the hippocampus area. The variables ambulation (A), stereotypy (E) and time ofpermanence (TP) in the central area of the box for 60? (open field); number of entries (NE) (times the rat passed from the white zone tothe black zone), TP in the white zone at 15? (light-dark test); and TP on the rotating roller (TPRG) were statistically studied with amixed model for longitudinal data (significant: P