EVALUATION OF COGNITIVE DISORDERS IN SPONTANEOUSLY DIABETIC RATS (eSS) AT DIFERENT AGES

PALMA NICOLÁS; CHOLICH VALERIA; FUNES ALEJANDRINA; ALLASIA BELÉN; CHAPO GUSTAVO; PACCHIONI ALEJANDRA; QUINTANA ALEJANDRA

ROSARIO

Congreso; XX Congreso y XXXVIII Reunión Anual de la SOCIEDAD DE BIOLOGÍA DE ROSARIO; 2018

SOCIEDAD DE BIOLOGÍA DE ROSARIO

Diabetes Mellitus type 2 (DM2) produces metabolic disorders that can induce cognitive dysfunctions. For instance DM2 is associatedwith memory deficit in elderly patients. The CIPREeB breeds spontaneously diabetic rats called eSS (e-Stilmann Salgado). TheeSSrats show increasing levels of glycemia (G), cholesterol (Col) and triglycerides (Tg) after postnatal day (PND) 200. Our main goalwas to evaluatethe progressive impactof DM2 on the cognitive abilities of eSS rats.TwelveeSS and 12 Wistar non-diabetic controlmalerats, were tested approximately every 30 days, between PND 80 and 280. The Novel Object Recognition Test was applied to evaluateshort-term (STM) and long term (LTM)memory. The test has three phases: 1) familiarization, where rats explore two identical objects;2) STM, one hour later rats explore an object from the familiarization phase (control object, CO)and a novelobject (NO) ; and 3) LTM,on the next day rats explore the CO(which is maintained) and a new NO. Each stage of the test lasts 3 min and was recorded toevaluateanimal?s behavior. The exploration time (t) foreach animal was the primary measure. Then, data were converted to arecognition index [tNO / (tNO + tCO)] to evaluate STM and LTM. A recognition index below 0.5 impliesmemory loss. A mixedgeneral linear model was fitted for longitudinal datato run the statistical analysis. It was considered statistically significant when p