EFFECT OF THE USE OF GASTRIC PROBE TO INDUCE HEPATIC PRENEOPLASIA ON THE PULMONARY HISTOLOGY OF eSS AND WISTAR RATS AND ITS EVOLUTION THROUGH TIME

ROSSI AGUSTINA; VALENTI JOSE; PISANI GERARDO ; BIDEGORRY MATIAS; PALMA NICOLÁS; LUGANO MARIA CRISTINA; QUINTANA ALEJANDRA

Congreso; XIX Congreso y XXXVII Reunión Anual de la SOCIEDAD DE BIOLOGÍA DE ROSARIO; 2017

SOCIEDAD DE BIOLOGÍA DE ROSARIO

The administration of toxins by gavage in rats can cause respiratory problems and/or animal?s death by aspiration. A model to inducehepatic preneoplasia (HP) to correlate it with diabetes, uses the promoter 2-acetylaminofluorene (2-AAF, by gavage). We analyzed thecollateral effect of 2-AAF administration by gavage, on eSS (spontaneously diabetic) and Wistar (W, controls) rat lungs, after theinduction of HP (125 d) and after 3 months after finishing the treatment (215 d), when the Diabetic Syndrome in eSS was declared (n=6per line). Rats received 2-AAF (20 mg / kg body weight, vehicle: corn oil, volume: 0.5 mL) as a promoter by gavage, 4 days per week,for 3 weeks. At the end of the induction (EI), 3 rats were euthanized from each line and the livers and lungs were removed. They werefixed in 10% v/v formaldehyde and histologically processed. Lung sections were stained with hematoxylin-eosin. Livers wereimmunohistochemically labeled to detect PH. After 3 months (3M), the remaining animals were euthanized. All rats developed PH.GrupoWEI: mild bronchitis with active leukocytic infiltrate. GrupoeSSEI: interstitial leukocytic infiltrate near the bronchioles and in theinterstitium, isolated nodular accumulations of macrophages and lymphocytes. At 3M, GroupW: bronchial obstruction due to necroticmaterial with polymorphonuclear infiltrate and lipophages, foci of bronchopneumonia due to aspiration and multifocal emphysema. After3M, GrupoeSS: interstitial lymphocytic infiltrates and few lipophages around bronchi. Both rat lines suffered bronchoaspiration of 2-AAF promoter agent and the vehicle, being milder in Group eSS. We concluded that the administration of 2-AAF by gavage producesserious lesions on lungs that worsen with time even after stopping 2-AAF administration in both lines of rats.