Prolactin modulation of hepatic ABC transporters in a murine model of the obesity. Study of Signal transducer and activator of transcription 5 (STAT 5) role

SEDLMEIER MARÍA GUILLERMINA; CERÉ LUCILA INÉS ; MARINOCHI MARÍA VIRGINIA; CATANIA VIVIANA ALICIA ; RONCO MARÍA TERESA ; FRANCÉS DANIEL ELEAZAR

Reunión virtual

Congreso; Annual Meeting 2021. Discovering the physiological gears from evolution to Translation October 20-22, 2021; 2021

Sociedad Argentina de Fisiología (SAFIS) y Asociación Latinoamericana de Ciencias Fisiológicas (ALACF )

Obesity is a metabolic disease characterized by alterations in serum levels of different hormones such as prolactin (PRL). Previously, we reported that mice fed with 40% High-Fat Diet (HFD) displayed elevated plasma levels of PRL that were associated with an increased canalicular expression of the ABC transporters Breast Cancer Resistance Protein (Bcrp) and P-glycoprotein (P-gp), since using bromocriptine (Brc, inhibitor of PRL release) protein expression of transporters were decreased. Also, previous studies have shown that PRL was able to induce transcription of basolateral hepatic transporters and its cognate receptor (Prlr) through activation of STAT5. We aimed to evaluate the mechanism involved in PRL modulation of canalicular transporters in vivo and in vitro. Five-week-old C57BL/6 mice were fed with regular chow diet (Control) or a HFD for 16 weeks. Another set of animals were injected with Brc 4 mg/kg or vehicle during three days. Data were expressed as percentage of Control±s.e.m. Significance was determined by Student's t-test. Nuclear STAT5 protein expression was increased in HFD (250±19%; n=4; p