EFFECT OF STRESS EXPOSURE IN THE PROGRESSION OF DIABETES. PARTICIPATION OF THE MICROBIOTA

RUBINSTEIN MR; WALD MR; GENARO AM.

Virtual

Congreso; LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2020

Sociedad Argentina de Investigación Clínica

Type 1 diabetes (T1D) is an autoimmune disease characterized by impaired insulin secretion. Recently, there was an increase in T1D suggesting the participation of environmental factors in its development. It is well known that the microbiota can modulate the immune system and multiple studies have shown a decrease in the microbial diversity prior and after the development of the T1D, but the mechanism involved remains unknown. In addition, it has been recognized the contribution of psychosocial factors in T1D. We have previously shown that chronic stress worsens the progression of T1D and multiple studies have shown that stress can modify the microbial composition. The aim of this project is to determine participation of the microbiota and stress on the development and progression of T1D and the mechanisms involved. To induced diabetes, we treated male BALB/c mice with multiple low doses of streptozotocin (stz) and then, the animals were subject to chronic mild stress (CMS) by a daily application of different mild stressors. Fecal samples were collected and genomic DNA was extracted. 16s Bacteriodetes and 16s Firmicultes (most abundant component of the microbiota) were measured by qPCR using specific primers. We found an increase in 16s Bacteroidetes after CMS exposure in stz treated mice (DIAB + CMS group) compared to all the other treatments (Ctrol, CMS and DIAB groups. One-way ANOVA, p