FRUCTOSE-ENRICHED DIET DECREASES PROTEIN EXPRESSION AND ACTIVITY OF INTESTINAL MRP2

ZECCHINATI, FELIPE; TOCCHETTI, GUILLERMO N.; BARRANCO, MARÍA M.; DOMÍNGUEZ, CAMILA J.; ARANA, MAITE R.; MOTTINO, ALDO D.; GARCÍA, FABIANA; VILLANUEVA, SILVINA S. M.

MAR DEL PLATA

Congreso; REUNIÓN CONJUNTA SAIC SAI SAFIS 2018; 2018

Increased intake of fructose in humans and laboratory rodents is known to be a risk factor for the development of metabolic disorders as insulin resistance, metabolic syndrome (MS) and type 2 diabetes as well as cardiovascular diseases. Mrp2 and phase II biotransformation enzymes, acting as intestinal biochemical barriers, strongly influence the bioavailability, and hence the efficacy and safety, of orally administered drugs. Aim: to evaluate the effect of the administration of 10% fructose (F) in drinking water to male Wistar rats for 8 weeks on jejunal Mrp2 expression, by western blot, and on its activity, by using the everted intestinal sacs model. Control rats (C) received tap water. Results: fructose consumption led to high plasma triglycerides and insulin levels, as well as insulin resistance and glucose intolerance in F vs C (p