UNDERSTANDING THE C4 PHOTOSYNTHESIS IN SETARIA VIRIDIS BY A PROTEOMIC APPROACH.

TONETTI, P.; GERRARD WHEELER, M.; MARGARIT, E.; ANDREO, C.S.; CALACE, P.; FIGUEROA, C.; SAIGO, M.

Mendoza

Congreso; SAIB-SAMIGE 2020-LVI Reunion Anual SAIB; 2020

Currently, the study of C4 photosynthesis is receiving great attention worldwide given the high demand for efficient systems in the production of biomass. The plants that perform C4 photosynthesis have a higher productivity per crop area related to an optimized use of water and nutrients. This is achieved through the introduction of a series of anatomical and biochemical features that allow the concentration of CO2 around Rubisco. C4 plants partition the photosynthetic reactions between two cell types, they initially fix the carbon to C4 acids within the mesophyll cells (MC) and then transport these compounds to the bundle sheath cells (BSC), where they are decarboxylated so that the resulting CO2 is incorporated into the Calvin cycle (CC). This work describes the activities focused on the comparative analysis of the proteins present in MC and BSC of S. viridis, a C4 model system close relative of several major feed, fuel, and bioenergy grasses. First, we prepared enriched preparations of the two main cell types MC and BSC and we obtained the total proteins by a method based on phenol extraction. Since the samples reached a satisfactory quality, they were sent for analysis to the protein identification service by mass spectrometry. With the obtained data, we carried out the statistical analysis and compared the abundances of the different proteins identified in each cell type. It was observed that 946 proteins are differentially expressed in one condition or another (p-value