PRENATAL HYPERANDROGENIZATION ALTERS LIPOGENESIS AND CHEMERIN IN GONADAL ADIPOSE TISSUE IN A MURINE PCOS MODEL

SILVANA FERREIRA; GISELLE ADRIANA ABRUZZESE; ALICIA BEATRIZ MOTTA; MARÍA JOSÉ FERRER; AIME FLORENCIA SILVA

Virtual

Congreso; 18 th Annual Meeting of Androgen Excess and PCOS Society.; 2020

Androgen Excess and PCOS Society

Prenatal androgen excess is considered one of the main factors contributing to Polycystic Ovary Syndrome (PCOS) development. Although it has been suggested that PCOS women have aberrant adipose tissue morphology and function, little is known about its role and alterations. This study aimed to evaluate the effect of prenatal hyperandrogenism on the main lipogenesis-related enzymes and two adipokines, chemerin, and adiponectin, in gonadal adipose tissue of rats during adult life (90 days of age).Pregnant rats received subcutaneous injections of 1mg of testosterone from day 16 to day 19 of pregnancy. Another group received subcutaneous injections with only a vehicle (corn oil). Female rats from hyperandrogenized mothers were assigned as the prenatal hyperandrogenized group (PH group), and the offspring from mothers that received a vehicle only formed the Control group. The female offspring of the PH and Control groups were characterized according to the estrous cycle from days 70 to 90. The PH group showed two phenotypes: irregular ovulatory phenotype (PHiov) and anovulatory phenotype (PHanov). We quantified, by qPCR, the gene expression of the main enzymes involved in lipogenesis (Acaca, Acacb, Fas) and the adipokine, chemerin. The protein expression of Peroxisome proliferator-activated receptors (PPARs), gamma and alpha (crucial factors in lipid metabolism), and adipokines, chemerin, and adiponectin (associated with metabolic disorders) were quantified by Western Blot. Besides, the triglyceride content in adipose tissue was quantified by Thin-layer chromatography.We found that the mRNA levels of Acaca (p