4-Methylumbelliferone promotes an antitumor immune response by improving the recognition of cancer stem cells and modulating the profile of antigen presenting cells.

RODRÍGUEZ M ; ONORATO A; DOMÍNGUEZ L; CANTERO M; FIORE E; BAYO J; GARCIA M; ATORRASAGASTI C; MALVICINI M; MAZZOLINI G

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencia (SAIC SAFE SAB SAP); 2019

Tumor microenvironment involves the interaction of different components including tumor cells, cancer stem cells(CSCs) and immune cells, such as macrophages(Mø) and dendritic cells(DCs). These interactions could promote tumor immune escape, growth and dissemination. Previously, we demonstrated that 4-Methylumbelliferone(4Mu) decreases the expression of the ?not eat me? molecule CD47 and facilitates phagocytosis of CSCs by Mø, supporting an antitumoral response in a murine model of hepatocellular carcinoma(HCC). The aims of this work were to evaluate whether the effects that 4Mu exerted on CSCs also enhance phagocytosis by DCs, analyze the role of immune response in the antitumoral efficacy observed in tumors treated with 4Mu, and study changes in the Mø profile. First, we performed a phagocytosis test using bone marrow derived-DCs from C3H mice. As a result, Hepa129 cells treated with 4Mu were more phagocytated by DCs than non-treated cells(p