Antifungal effect of ethanolic extracts from native plants of La Rioja

SAYAGO M ; DÁVILA E; LEYVA J; BENAVENTE A; BARBIERI N

Tafí Viejo, Tucumán

Jornada; XXXVI ANNUAL SCIENTIFIC MEETING, TUCUMAN BIOLOGY ASSOCIATION; 2019

In La Rioja olive and grapevine crops are affected by phytopathogenic fungi such as Verticillium dahliae and Phaeoacremonium parasiticum, which decrease their quality and yield. Our objective was to study the ethanol extracts (EE) antifungal effect of La Rioja native plants on the phytopathogens: V. dahliae and Pm. Parasiticum.The EE were obtained by maceration of dry plant material from Zuccagnia punctata, Clinopodium gilliesii, Lippia turbinata, Lippia integrifolia, Argemone subsiformes, Caesalpinia gilliesii and Senecio nutans. The percentage of mycelial growth inhibition was determined V. dahliae (7 and 9 days) and Pm. parasiticum (7, 9 and 12 days). Benomil was used as a reference antifungal. The results were expressed as MIC (minimum inhibitory concentration of 100% of mycelia growth).Z. punctata EE was able to inhibit V. dahliae growth at 7 and 9 days (MIC=1.5 mg/mL). C. gilliesii EE presented antifungal activity against V. dahliae at day 7 (MIC=2.5 mg/mL) and at day 9 (MIC=3 mg/mL).Z. punctata EE was effective in inhibiting the growth of Pm. parasiticum at day 7 (MIC=1.5 mg/mL) and at days 9 and 12 (MIC=2 mg/mL). Another extract that showed inhibitory activity against Pm. parasiticum was L. turbinata at day 7 (MIC=2 mg/mL) and at day 9 (MIC=3 mg/mL).The antifungal activity of C. gilliesii and L. turbinata EE decreases over time, so they could have a fungistatic effect.The EE Z. punctata resulted in the highest inhibitory potential of the two phytopathogens. The inhibitory activity of this EE on V. dahliae was kept at steady time rates, however, on Pm. parasiticum fungistatic effect was produced.