PLANT REGENERATION THROUGH ANTHER CULTURE IN APOMICTIC CULTIVARS OF BUFFEL GRASS

CARLONI, E.; GRUNBERG, K.; GRIFFA, S.; RIBOTTA, A.; LÓPEZ COLOMBA, E.; TOMASSINO, E.; LUNA, C.; BIDERBOST, E.

Huerta Grande Córdoba

Jornada; XVII Jornadas de la Sociedad de Biología de Córdoba.; 2009

Sociedad de Biología de Córdoba

PLANT REGENERATION THROUGH ANTHER CULTURE IN APOMICTIC CULTIVARS OF BUFFEL GRASS Carloni E, Grunberg K, Griffa S, Ribotta A, López Colomba E, Tommasino E, Luna C, Biderbost E. Instituto de Fitopatología y Fisiología Vegetal (IFFIVE-INTA), Camino 60 cuadras Km 5˚. CP X5020ICA. Córdoba, Argentina. Buffel grass is a grass used as forage in the Argentinian North West. It reproduces by apomixis thus generate variability by conventional breeding methods is difficult. Various biotechnology techniques to reduce or increase the level of ploidy such as anther culture can be combined to develop strategies for improvement. The aim of this study was to evaluate the response to androgenesis through anther culture in three cultivars of Buffel grass. To induce embryogenic calli, anthers containing microspores at the uninucleate stage of cvs. Biloela, Americana and Bella were cultured in darkness in Petri dishes, with basal medium Murashige and Skoog (MS), 3% (w/v) sucrose and 0.7% (w/v) of agar. The medium was supplemented with different combinations of 2,4-dichlorophenoxyacetic (2,4-D) 2, 4 and 6 mg / l 2 +0.5 4 +0.5 6 +0.5 mg / l 2,4-D +6 -benzylaminopurine (BAP), respectively, at 25ºC, pH 5.8. Moreover, the inflorescences were subjected to different pretreatments: cold (5 and 7 days) and heat shock (40°C 30" + 10°C 30"). Embryogenic calli were transferred to germination medium (MS +1 mg / l (BAP) +0.1 mg / l naftalenacetic acid (NAA), 0.7% (w / v) agar (pH 5.8). Green plant regeneration from anther-derived embryogenic calli was achieved in Biloela and Bella. The highest percentage of plants was obtained on cv. Bella in cold pretreatments with 5 days (1.7%) and heat shock (1.7%). Plant regeneration was linked closely to number of produced embryogenic calli and has allowed regenerating green plants in two of the three cultivars of grass Buffell used.