IDENTIFICATION OF CRISP1 PROTEIN IN THE FEMALE REPRODUCTIVE TRACT

ERNESTO JI; CALB D; WEIGEL MUÑOZ M; COHEN DJ; CUASNICU PS

Buenos Aires

Jornada; XI Jornadas Multidisciplinarias de la Sociedad Argentina de Biología (SAB); 2009

Sociedad Argentina de Biología (SAB)

A92 ABSTRACTS BIOCELL, 34(2) 201029.IDENTIFICATION OF CRISP1 PROTEIN IN THE FEMALEREPRODUCTIVE TRACTErnesto JI, Calb D, Weigel-Muñoz M, Cohen DJ, Cuasnicu PS.IByME-CONICET. Bs As, Argentina.CRISP1, CRISP2, CRISP3 and CRISP4 are members of the CRISP(Cystein RIch Secretory Proteins) family which have been mainlycharacterized in the male reproductive tract. As little informationexists on these proteins in females, in the present work we investigatedthe expression of CRISP1 protein in the female reproductivetract. The presence of CRISP1 mRNA was evaluated in uterus,ovary, oviduct and cumulus cells from adult rats by RT-PCR followedby sequencing of the PCR products. Results showed the presenceof CRISP1 mRNA in all the samples tested. To study the expressionof these messengers, protein extracts from uterus, ovaryand oviduct were analyzed by Western blot using a specific antibodyagainst CRISP1. In agreement with our observations, CRISP1protein was detected in the three tissues evaluated. In addition tothis, cumulus cells incubated with anti-CRISP1 and analyzed byindirect immunofluorescence exhibited a clear fluorescent labelingnot observed in cells incubated with a control antibody. Together,these results confirm the expression of CRISP1 in the femalereproductive tract opening the possibility of new roles forthis protein within this tract.