The aqueous extract of Smilax camprestris inhibited the osteoblast secretion of IL-6 and decreased the osteoblast-induced osteoclast differentiation

MANGONE FRANCO; SALAVERRY LUCIANA; SOTELO AGUSTINA; MACCHI ROSARIO; PARRADO CECILIA; BESSONE MARCO; REY-ROLDAN ESTELA; CASTRO MARISA; CANELLADA ANDREA

Congreso; Reunión Anual de Sociedades de Biociencias SAI SAI SAFIS 2020; 2020

Background: Smilax campestris is a medicinal plant that has been used traditionally to treat inflammatory diseases. During bone remodelling, osteoblast cells secrete pro-inflammatory mediators that activate osteoclast differentiation and activity. This process is exacerbated in the absence of oestrogen. Aims: to determine whether the aqueous extract of S. campestris (SME) inhibits the osteoclast differentiation of RAW 264.7 cells induced by conditioned medium from inflammatory MC3T3-E1 osteoblast precursors, and the mechanism underneath. Methods: MC3T3-E1 cells were incubated with SME (10, 100, 1000 ng/mL) or oestrogen (E2 10-8, 10-7, 10-6 M) for 24 h, and then treated with IL-1β (2.5 ng/mL) for 24 h. After treatments, the cells were cultured for 24 h without stimuli to obtain conditioned media (CM). IL-6 concentration in CM was measured by ELISA. RAW cells were incubated with CM for 7 days and then tartrate resistant acid phosphatase positive (TRAP+) cells and MMP-9 activity were determined by histochemistry and gel zymography, respectively. Results: SME decreased IL-6 secretion induced by IL-1β in MC3T3-E1 cells, being this effect dose-independent (% inh= 91.83.8 at 10 ng/mL, p