INVESTIGADORES
SVARTZ Gabriela Veronica
congresos y reuniones científicas
Título:
EVALUACION DE LOS EFECTOS LETALES, ESTRÉS OXIDATIVO Y GENOTOXICIDAD EN LARVAS DE RHINELLA ARENARUM EXPUESTAS AL INSECTICIDA DIMETOATO
Autor/es:
MERCEDES ACQUARONI; JULIETA PELUSO; GABRIELA SVARTZ; CAROLINA ARONZON; CRISTINA PÉREZ-COLL
Lugar:
Virtual
Reunión:
Congreso; XXXVII Congreso Interamericano virtual de Ingeniería Sanitaria y Ambiental de AIDIS; 2021
Institución organizadora:
Asociación Interamericana de Ingeniería Sanitaria y Ambiental
Resumen:
There is a great concern worldwide about the global decline of amphibians particularly by agrochemical pollution. The aim of this study was to assess acute lethal effects and sublethal as oxidative stress and genotoxicity of a commercial formulation of the insecticide dimethoate in Rhinella arenarum larvae, using biomarkers of sublethal effects. The 24 h and 96 h-LC50 values of dimethoate to R. arenarum were 48.81 and 38.86 mg L-1 while the 96 h-No Observed Effect Concentration (NOEC) value was 20 mg L-1. For biomarker of sublethal effect assays, R. arenarum larvae were exposed to 1.25, 2.5 and 5 % of the 96 h-NOEC- (0.25, 0.5, 1 mg L-1 dimethoate respectively). After 96 h of exposure, inhibition of catalase (CAT) and glutathione S-transferase (GST) activities were registered. Also, an increased superoxide dismutase (SOD) activity was observed in larvae exposed to the highest concentration (1 mg L-1). Lipid peroxidation by increased TBARS levels in larvae exposed to 0.5 and 1 mg L-1 was detected. No differences in micronuclei frequency between treatments and negative control were observed. These results demonstrate the oxidative toxicity of dimethoate at sublethal concentrations in Rhinella arenarum larvae. The disruption of defense mechanisms may contribute to a deleterious impact on amphibian populations from habitats exposed to this organophosphorus insecticide.