CONICET | Buscador de Institutos y Recursos Humanos

Seasonality in the reproduction of water buffalo is one of the main problems of breeders to have a constant production. Some authors have been suggested that the use of out of breeding season ovulation induction programs, might help to overcome this problem. The objective of this work was to evaluate the effect over pregnancy rates the application of an ovulation synchronization protocol designed for out of breeding seasons. This work was performed in September of 2018, during out of breeding season in a farm located in Corrientes, Argentina. 26 adult normal females of similar weight and parity were synchronized, using progesterone, eCG, prostaglandin and estradiol cipionate protocol. Ten days before the starting the synchronization protocol half of the animals were treated with melatonin implants at a dose of 18mg/50 kg of live weigh. At the beginning of the protocol blood sample to determine antimüllerian hormone (AMH) was taken. Inseminated by the same technician with frozen semen of Murrah bulls of proved fertility. Pregnancy rates were compared with those obtained during breeding season artificial insemination protocols in the same farm. Data were analyzed using comparison of proportions, using Tukey test and statistical difference were considered p>0.05. 9 of the 26 females became pregnant (34.61%) compared to (52.5%) during breeding season, with no statistical difference (p=0.2076). No difference in AMH levels were found between pregnant and non-pregnant females, 245.5 vs 238.7 pg/ml (p=0.1496). No effect of male in pregnancy rates were observed. This result shows comparable results in terms of pregnancy rates in out of breeding?s season allowing farmers to implant this strategy as part to satisfy market demand´s. As reported by others they are no effect of ovarian reserve analyzed through AMH levels over pregnancy rates.

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