PERSONAL DE APOYO
GOMEZ CARRILLO Manuel
congresos y reuniones científicas
Título:
EARLY SPREAD OF HIV-1 B/F RECOMBINANT INTERSUBTYPE IN CHILDREN BORN TO INFECTED MOTHERS IN ARGENTINA
Autor/es:
GÓMEZ CARRILLO M,; AVILA, M;; PANDO, M;; MARTÍNEZ PERALTA, L;; RUSSELL, K;; CARR, J.K.
Lugar:
Buenos Aires
Reunión:
Conferencia; The 1st IAS Conference on HIV Pathogenesis and treatment; 2001
Institución organizadora:
International AIDS Society
Resumen:
Introduction: In Argentina, the first AIDS case was detected
in 1982. HIV-1 sequences reported during early ´90 showed
a predominance of subtype B. Since 1996 subtype F and
recombinants B/F were reported and a high proportion of B/F
recombinant was observed in 2000 in different populations.
In order to elucidate the possible change in the molecular
patterns of HIV-1 epidemic in Argentina, sequences obtained
from proviral DNA present in PBMC samples from infected
children born before 1995 were compared with sequences of
virus obtained in children born in 1998-2000. Methods: A
total of 25 PBMCs samples from children born to HIV infected
mothers in 1984 to 1989 (14 children) and 1995 to 2000 (11
ch.) were collected. The selected region for sequencing was
previously found as a common break-point site for most of
B/F recombinants in Argentinean sequences. A nested PCR
strategy was performed in order to amplify a 600-bp
fragment containing the break point (position 5967 to 5570 in
HXB2 reference sequence). Sequencing was performed in an
automated sequencer (Applied Biosystems 3100). A multiple
alignment of the sequences with selected reference
sequences of subtype B and subtype F was constructed.
Phylogenetic trees were made using Neighbor Joining and
Treetool. Results: B/F recombinant sequences were found in
6/14 children born between 1984 and 1989: 1 born in 1986
and 5 born in 1987. The remaining 8 children born in this
period showed a B pattern for the 600 bp fragment. Among
children born after 1995 4 were B and 7 were B/F
recombinants. Two siblings, born in 1985 and 1989, showed
the same B pattern and clustered together. Conclusions: A
high percentage of B/F recombinants were observed as from
1986/87, when only 90 AIDS cases were reported in
Argentina in that same period. Data shown in this population
are suggesting that the recombinants had an early spread in
Argentina and are probably predominant in the present time.
Argentina and are probably predominant in the present time.