INVESTIGADORES
BOUVIER Leon Alberto
congresos y reuniones científicas
Título:
Alternative approaches for control of gene expression in trypanosomatids.
Autor/es:
NIEMIROWICZ, GABRIELA T.; ALVAREZ, VANINA E.; BOUVIER, LEÓN A.
Lugar:
Rockville, Maryland
Reunión:
Congreso; XXXI Molecular Parasitology Meeting.; 2020
Institución organizadora:
Genetics Society of America
Resumen:
Regulatable gene expression in trypanosomatids relies on pre-establishing cell lines modified to express exogenous regulatory elements such as bacterial repressors and polymerases and therefore, inducible experimentation is frequently restricted to few laboratory strains. Moreover, extending these methodologies to other strains usually requires several additional transfection steps as well as lengthy periods in culture thus affecting the natural wild type properties sought after in field isolates.Here we explored alternative strategies for inducible transgene expression in Trypanosoma cruzi and Trypanosoma brucei. We studied the drug dependent modulation of GFP mRNA stability by insertion of a cis-acting riboswitch. This element consists of a Schistosoma mansoni hammerhead ribozyme fused to a tetracycline aptamer. Reporter GFP expression could be downregulated by tetracycline addition, in T. cruzi epimastigotes as well as in procyclic T. brucei trypomastigotes. Thus, our results would suggest that these riboswitches could be used as alternative tools to study essential genes in trypanosomatids.