TGF- Β1 IMPLICATIONS ON LUMINAL-MYOEPITHELIAL DIALOGUE IN BREAST CANCER

SCIACCA M ; ZAMBRANO M ; MARINO L ; EIJAN ANA MARIA.; LODILLINSKY C

MODALIDAD VIRTUAL

Congreso; Reunion Conjunta de Sociedades de Biociencias SAIC SAI SAFE; 2020

SAIC SAI SAFE

The mammary gland duct is composed by an internal cell line formed by luminal cells (LEP) surrounded by an external one of myoepithelial cells (MEP). It is still unknown how these cells contribute to the progression of ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC). Some works suggest that TGF-β1 pathway may be involved in epithelial-mesenchymal transition and confer stem cell properties to DCIS cells. This implies that this pathway might be a potential pathological mechanism which drives the progression of DCIS into IDC.The cellular model LM38 consists of three cell lines: LM38-LP (MEP and LEP), LM38-HP (LEP) and LM38-D2 (MEP). Previously, we described that only the bi-cellular LM38-LP cell line was able to develop in situ tumors after intraductal injections, suggesting that cell interaction could confer an advantage for tumor formation and progression. Moreover, we showed that treatment with conditioned medium of LM38-D2 induced viability on LM38-LP cells.The analysis of TGF-β1 expression in the LM38 model showed higher levels of TGF-β1 mRNA in LM38-D2 compared to LM38-LP (qPCR, p