EFFECTS OF THE ANGIOTENSIN II TYPE 2 RECEPTOR (AT2R) AGONIST, COMPOUND C21, ON THE EXPRESSION OF APELIN AND ITS RECEPTOR APJ IN THE POST-ISCHEMIC KIDNEY. POSSIBLE INTERACTION BETWEEN RENIN-ANGIOTENSIN AND APELINERGIC SYSTEMS.

MOLINAS, SARA M.; FUSSI M. FERNANDA; LAROCCA, CECILIA; MONASTEROLO, LILIANA A.

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias SAIC, SAFIS, SAI 2020; 2020

SAIC-SAI-SAFIS

In previous experiments we observed that pretreatment with theAT1R antagonist, losartan, or the AT2R agonist, C21, preventsglomerular filtration drop, loss of sodium reabsorption, and loss ofbrush border in a renal ischemia/reperfusion (I/R) model. On the otherhand, we have found that I/R decreases the expression of the adipokineapelin; and in turn the pretreatment with apelin prevents theimpairment of proximal tubular transport and has anti-inflammatoryeffects in this model. We also demonstrated that losartan preventsthe decreased expression of apelin and its receptor APJ. The aimof this study was to evaluate the effects of the AT2R agonist, C21,on apelin and APJ mRNA levels in an I/R model. Male Wistar rats(5-6 per group) underwent unilateral renal ischemia for 40 min followedby 24 h of reperfusion (I/R) or sham surgery (C). C21, 0.3mg/Kg/d i.p., or its vehicle was administered for two days prior to I/R.mRNA levels were analyzed by qRT-PCR. Apelin mRNA levels decreasedin I/R. This decrease was prevented with C21 pretreatment(%change fold C: C+C21=+15; I/R= -92*; I/R+C21= -12#; *p