CHARACTERIZATION OF SUBPOPULATIONS OF MONOCYTES ACCORDING TO THE STAGE OF CHRONIC CHAGAS DISEASE. MODULATORY ROLE OF FENOFIBRATE

PIERALISI, AZUL VICTORIA; NILDA PRADO; MORI ANA; FEDERICO N PENAS; ÁGATA CAROLINA CEVEY; MARÍA JIMENA RADA; GERARDO ARIEL MIRKIN; GAGLIARDI J; GOREN, NORA

Congreso; Reunión Anual De Sociedades de Biociencia 2020; 2020

Sociedad Argentina de Investigación Clínica (SAIC), SAI, SAFIS

Chronic cardiomyopathy is the most important clinical manifestationof Chagas disease. We previously demonstrated in a murine infectionmodel that fenofibrate (Fen), PPARα agonist, controls inflammation,prevents fibrosis and improves cardiac function. Consideringthat different subpopulations of monocytes (mono) are involvedin the progression of cardiomyopathy, we decided to characterizethese subpopulations according to the stage of the disease and toevaluate if in vitro treatment with Fen drives mono towards a tissuerepair profile. Seventeen healthy individuals (HI) and 16 patientswith positive serology for Chagas, without (E0) and with chronicChagas cardiomyopathy (CCC) were included. Peripheral bloodmononuclear cells were purified, cultured and stimulated or not withT. cruzi (Tc) lysates (10μg / ml), and treated with Fen (100μM). Underbasal conditions, CCC showed a higher percentage of classicalmono than E0 (p