Endocrine disrupting environmental pollutans Hexachlorobenzene and Chlorpyrifos induce endometriosis associated angiogenesis

CEBALLOS L; MIRET N; PONTILLO CA; ZÁRATE L; NÚÑEZ M; ÁLVAREZ L; RANDI A; CHIAPPINI F

Congreso; Reunión Anual de Sociedades de Biociencias; 2020

Endometriosis is a frequent and chronic illness which is defined by the existence of endometrial tissue outside the uterus. Angiogenesis is critical in endometriosis progression, is a complex process involving endothelial cell migration, proliferation, tube formation, and survival. Vascular endothelial growth factor (VEGF) is a powerful angiogenic factor, which activates downstream effectors such as ERK1/2. This signaling pathway is involved in endothelial cell proliferation and migration. Endocrine-disrupting environmental pollutants are thought to play a role in the development of this disease. Hexachlorobenzene (HCB) is an organochlorinated pesticide that increases microvessel density and VEGF levels in a rat endometriosis model. Chlorpyrifos (CPF) is an organophosphate insecticide that acts as an Endocrine Disruptor. The present study examined the effect of HCB and CPF on endometriosis angiogenesis in vitro. T-HESCs cells (stromal endometrial cell line) were exposed to HCB (0.005, 0.05, 0.5 and 5 μM) and CPF (0.05, 0.5, 5 and 50 μM) or vehicle for 48h, and the conditioned media were then used to stimulate EA.hy926 endothelial cells. The results showed that HCB (0.005, 0.05 μM) and CPF (0.5, 5, 50 μM) induced VEGF secretion (p