EARLY EVENTS OF INNATE IMMUNITY ARE MODIFIED BY THE NANOFORMULATION OF VACCINE COMPONENTS

MARIN C, RUIZ MORENO FN, PASCUAL MM, PALMA SD, ALLEMANDI DA, MORÓN VG, PISTORESI MC, MALETTO BA

Tucuman

Congreso; LXVII REUNIÓN SAI; 2019

Subunit vaccines are often poorly immunogenic and require a strong adjuvant. Therefore, new adjuvant strategies are demanded to develop new vaccines. We formulated OVA and CpG-ODN with a nanostructure (Coa-ASC16) formed by self-assembly of 6-O-ascorbyl palmitate. This nanoformulation (OCC) elicited strong humoral and cellular (CD4+ and CD8+) responses, which are superior to those induced by a solution of OVA and CpG-ODN (OC). Nonetheless, some characteristics about action mechanism of Coa-ASC16 remain to be elucidated. In this work we study some aspects about early immune events. Mice were subcutaneously immunized with OC or OCC formulation. Cytokines/chemokines were quantified in lymph nodes and serum at 0.33, 2, 24 and 48 hours post-immunization by LEGENDplex Mouse Inflammation Panel 13-plex. We further determined by flow cytometry, OVA and CpG-ODN cellular uptake in lymph nodes 24 hours post-immunization. Lymph nodes of OCC immunized mice showed higher levels of IFN-γ, IL-12p70, IL-1β, IL-10, IL-6, IL-27, IL-17A and IFN-β than mice immunized with OC (p