Erythropoietin and iron availability in the regulation of hepcidin in hepatic cells.

MALTANERI, R; CHAMORRO, ME; IOLSTER, J; ALVAREZ, G; NESSE, A; VITTORI, D

Congreso; LXV Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2020

Sociedad Argentina de Investigación Clínica

Maintenance of systemic iron (Fe) levels undergoes regulation by the erythropoietic demand, inflammation and Fe status. Liver hepcidin (Hep), a small peptide which binds the Fe exporter ferroportin thus inducing its internalization and degradation, is a key regulatory target for Fe homeostasis. Using cultures of the human hepatic cell line HepG2, we examined the ability of erythropoietin (Epo) to regulate Hep mRNA expression (real-time PCR). Hep levels were significantly reduced by exposure to Epo (160 ng/mL, 6 h), while its non-erythropoietic, carbamylated derivative cEpo failed to exert this effect (Control: 1, *Epo: 0.4±0.2, cEpo: 0.9±0.1; *P