Nitric oxide and cytokines levels after LPS challenge in rats: effects of dietary (-)-epicatechin.

FRAGA CG; GONZÁLEZ MAGLIO D; FISCHERMAN L; GALLEANO M

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2019

SAIC

Endotoxemia is responsible for numerous effects both at tissue and systemic level which can be related with the activation of inflammation signaling pathways. Those mechanisms are triggered by complex interactions and involve multiple cell types, which produce a variety of signaling molecules such as nitric oxide (NO) and cytokines, driving to cell and tissue damage and disfunction. Some polyphenols from diet have known anti-inflammatory effects and this work has the aim to investigate the role of a flavanol, (?)-epicatechin (EC), in modulating inflammatory response at different molecular and tissue levels. Sprague-Dawley male rats, pretreated for 4 days with control diet or with diets supplemented with 20 (low-level, LLE) or 80 (high-level, HLE) mg (−)-epicatechin (EC)/kg of body weight/day, were challenged with bacterial lipopolysaccharide (LPS) (i.p. 4 mg/kg BW) and euthanized 6 h post LPS administration. In LPS-treated endotoxemic animals, plasma GPT, GOT and LDH levels were slightly or non-affected. However, NO levels in blood, measured by electronic paramagnetic resonance, increased significantly, and HLE avoided 41% (p