INVESTIGADORES
LEIVA Natalia Lorena
congresos y reuniones científicas
Título:
Interplay between Rab11, Rab14 and FIP2 in Chlamydia trachomatis-infected cells.
Autor/es:
LEIVA N; CAPMANY A; GAMBARTE J; DAMIANI MT
Lugar:
Ciudad de Mendoza. Mendoza
Reunión:
Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB); 2012
Institución organizadora:
SAIB
Resumen:
Chlamydia trachomatis is a bacterial causative agent of major
sexually-transmitted diseases and blindness in humans. During its
development, this obligate intracellular bacterium resides and
multiplies in a modified vacuole called inclusion. is a bacterial causative agent of major
sexually-transmitted diseases and blindness in humans. During its
development, this obligate intracellular bacterium resides and
multiplies in a modified vacuole called inclusion. inclusion.
C. trachomatis subverts key eukaryotic proteins in charge of vesicular transport to
prevent its degradation by phagocytosis, and simultaneously, to
obtain nutrients from parasitized cells. Rab GTPases are master
controllers of intracellular trafficking pathways, which cycle
between a GTP-bound form (active) to a GDP-bound form
(inactive). The aim of this study was to investigate if Chlamydia subverts key eukaryotic proteins in charge of vesicular transport to
prevent its degradation by phagocytosis, and simultaneously, to
obtain nutrients from parasitized cells. Rab GTPases are master
controllers of intracellular trafficking pathways, which cycle
between a GTP-bound form (active) to a GDP-bound form
(inactive). The aim of this study was to investigate if Chlamydia Chlamydia
manipulates host Rab39-mediated vesicular trafficking. We
observed that Rab39a associated with the chlamydial inclusion
along its developing time. Rab39a colocalized with IncA and IncG,
two bacterial proteins, at the chlamydial inclusion membrane. We
determined that the recruitment of Rab39a is bacterial protein
synthesis-dependent. Furthermore, Rab39a recruitment to the
inclusions remains unaltered after treatment with drugs that
interferes with the cytoskeleton, such as nocodazole,
butanedionemonoxime and cytochalasin. Rab39a decorated
vesicles carrying sphingolipids and labeled with lysotracker in
infected cells. Furthermore, overexpression of Rab39a WT and its
positive mutant Rab39a Q72L increased the chlamydial inclusion
size. Therefore, these results suggest a potential role for Rab39 in
chlamydial infection outcome.