EVALUATION OF CIRCULATING LYMPHOCYTES SUBPOPULATIONS DURING THE GROWTH OF M-406 TRIPLE NEGATIVE MURINE MAMMARY TUMOR

DEL GIÚDICE ANTONELA; CAPITANI M. CELESTE; FUSINI MATÍAS E.; MAINETTI LEANDRO E.; SCHAROVSKY OLGA GRACIELA; RICO MARÍA JOSÉ; ROZADOS VIVIANA R.

Mar del Plata

Congreso; LXIV Reunión Anual de SAIC, LI SAFE, XXI SAB, XXXI SAP; 2019

Inbred mice models provide an interesting tool for identifying factors that control susceptibility to breast cancer. M-406 mammary adenocarcinoma appeared in an inbred CBi mouse. CBi- mice were artificially selected from CBi. Cells of the immune system play an important role in tumor development. In order to determine their participation on tumor growth in genetically different hosts, CBi, CBi- and F1 reciprocal hybrids (F1A: CBi x CBi- and F1B: CBi- x CBi) were s.c. challenged with M-406, tumors were measured, and blood samples were taken on days 0, 7 and 14 in CBi and F1 and on days 0, 5, 8 and 12 in CBi- mice. Circulating CD4+, CD8+, Treg and Th17 cells were quantified (flow cytometry). Tumors grew exponentially in 100% of CBi (susceptible) and F1 females and males mice. However, in CBi- (resistant) after a short period of growth, reaching the maximum size on day 8 (females) and 12 (males), 100% of tumors were rejected. CBi, F1A and F1B mice, did not differ in Tumor Volume Doubling Time (TVDT) for both sexes, while in CBi-, TVDT in males was higher than in females (Pday 14; (P