MODULATION OF THE GLUCOSE CONSUMPTION DURING THE IN VITRO MATURATION OF PORCINE COC. INSULIN-TRANSFERRIN-SELENIUM AND METFORMIN EFFECT

LUCHETTI, CG.; LORENZO, MS.; ELIA; TEPLITZ, G.; CRUZANS, PR.; GHERSA, J; LOMBARDO, DM.

Ciudad Autónoma de Buenos Aires

Otro; IV REUNIÓN CONJUNTA DE SOCIEDADES DE BIOLOGÍA DE LA REPÚBLICA ARGENTINA; 2020

Sociedad Argentina de Biología

The objective of this work was to assess the effects of the supplementation of porcine in vitro maturation (IVM) with metformin (M) and insulin-transferrin-selenium (ITS) on the glucose consumption of pig cumulus-oocyte complexes (COC). The pig is an excellent model to study the effects of the supplementation of defined media for oocyte IVM due to the metabolic and physiological similarities of this species with humans, in addition to the ease of obtaining a large number of COC from slaughterhouse ovaries. There is a low efficiency in the in vitro production of porcine embryos, mainly due to the inefficient IVM systems. Supplementation of the IVM medium with M and insulin showed to improve porcine embryonic development. In our laboratory, we saw that M+ITS increases nuclear maturation decreases oxidative stress and increases the viability of cumulus cells. Studying the mechanisms involved, we consider that glucose consumption may have an important role. Porcine COC obtained by follicular aspiration from slaughterhouse ovaries were selected according to quality and subjected to MIV 44-48 h in TCM-199 + porcine follicular fluid (10%) and FSH (30 µg / mL). The COC were randomly distributed in IVM drops supplemented with: Group M: M (10-4 M), Group ITS: ITS (1 μg/ ml), Group M+ITS: M (10-4 M) + ITS ( 1 μg/ ml) and Group C: without supplement. Glucose concentration was detected with the Glucose Liquid Plus kit (GT Lab, Rosario, Argentina) in media samples, before and after IVM, stored at -20 ° C until use (n = 4/ experimental group, 10 µL per determination). Glucose consumption was calculated as the difference between the concentration in the pre and post IVM media. The results (mg glucose/ dL) were analyzed using the Kruskal-Wallis test, and the Dunn test for differences between columns. Glucose consumption was increased in the ITS and M+ITS groups compared to the C and M groups (p