An integrated study of ecotoxicological endpoints in soils of an Argentine farming area after application of commercial glyphosate and chlorpyrifos to a soya field

CASABÉ, NORMA B.; PIOLA, LUCAS; FUCHS, JULIO S.; ONETO, MARÍA LUISA; GIMÉNEZ, ROSANA; PAMPARATO, LAURA; BASACK, SILVANA; MASSARO RUBÉN; PAPA, JUAN C; KESTEN, EVA

Holanda

Congreso; 16 SETAC Europe 16th Annual Meeting,; 2006

SETAC Europa

A RR soya field in a traditional farming area was divided into 3 blocks, each comprising 3 plots of 13 m x 5 m. At each block, randomly chosen plots were sprayed with commercial chlorpyrifos (CPF) (620 g ai/ha 30 days after seeding - t0), commercial glyphosate (GLY) (1,440 g ai/ha, 24 h after seeding and at t0), or water (control). Soil physicochemical analysis was performed. For laboratory bioassays using earthworms of Eisenia fetida andrei, soils were sampled at different times after t0. A battery of subcellular, cellular and whole organism endpoints was assessed. Lysosomal destabilization of coelomocytes resulted a sensitive biomarker. Comet assay showed damaged DNA with ClP. Enzymatic biomarkers in earthworm tissues were not modified. GLY reduced cocoons viability, decreasing the number of juveniles. Earthworms avoided soils treated with GLY. CPF and GLY treated plots showed a diminished alimentary activity after 50 days (field bait lamina test). Litterbag field assay showed no differences between plots. Some of the studied endpoints were modified at chemically non-detectable concentrations and might constitute an early warning of deleterious effects.